A Practical Guide to Good Pipetting Practice

Nail your volumes, protect your samples, and keep your hands healthy. This expert handbook from Rainin distills Good Pipetting Practice™ into clear, lab-ready recommendations, covering pipette selection, tip quality, technique, ergonomics, and instrument maintenance.

What’s inside:

• Pick the right tool for the job: When to use air-displacement vs positive-displacement pipettes (incl. viscous, volatile, hot/cold, foaming, or hazardous liquids), and when to move from single- to multi- channel to semi-automated pipettes

• Tip quality that protects your data: How fit, sterility, filtration, and low-retention properties impact accuracy, why contamination matters, and sustainability-minded options that cut plastic waste

• Technique that moves the needle: Forward vs reverse pipetting and the small fixes that add up e.g., correct immersion depth, steady aspiration speed, optimal angle, proper dispensing and pre-rinsing

• Ergonomics that prevent RSIs: Bench setup, posture, stretch routines, and low-force pipettes/tips to reduce strain and error

• Care, calibration, and compliance: Cleaning/decontamination guides, risk-based service intervals, and the latest ISO 8655:2022 essentials for testing/maintenance

Highlights from the handbook

• Clear tables that map liquid properties → recommended pipette type/technique (pages 14–15, 44–45)

• Visual quick guides for forward vs reverse pipetting and optimal pipetting posture (pages 36–39, 46–47)

• Side-by-side tip type comparison (standard, filtered, low retention, wide orifice, shielded, extended length, capillary piston) (pages 28–29)

• Practical cleaning/decontamination recipes for DNA/RNA, proteins, RNase, solvents, and radioactivity (pages 52–53)

Get this eBook and level-up your pipetting accuracy, reproducibility, and comfort—starting with your next run.