Automating Plate-based Expression: Drivers of Protein Production Performance

This resource highlights patterns, pitfalls, and protocol insights that you can adapt to improve the consistency, output, and automation of plate-based protein expression.

You’ll see where variability really comes from and which steps are more flexible than most protocols imply, including the robustness of different transfection methods.

Factors such as plate position effects and reagent handling variability can both impact reproducibility in 96-well expression workflows. In this poster, these factors are examined using data generated with the Expi293™ PRO Expression System for production of mAbs and fusion proteins.

Alongside these observations, the poster provides practical protocol tips and considerations at other stages of the protein production process—from plasmid DNA preparation and complexation through to cell addition and feeding.

What you’ll learn:
• Protocol-level insights to help you automate your system
• A clear view of the physical and biological forces shaping 96-well performance
• Actionable takeaways to help you improve reproducibility and yield

Get your free poster below and improve your plate-based expression workflow