FREE RNA PURIFICATION KIT
RNA Extraction from TRI Reagent® in 7 minutes without the Tedious Steps
Discover the most efficient method to recover total RNA including miRNA from TRI Reagent® bypassing chloroform, phase separation, or time-consuming alcohol precipitation steps.
The Direct-zol™ RNA Microprep Kit simplifies RNA extraction by allowing RNA to bind directly to a spin column (or magbeads) from samples lysed in TRI Reagent® or similar, removing the need for chloroform and other complex handling steps.
Faster, Simpler RNA Extraction, Without Phase Separation
The conventional RNA extraction method relies on phase separation and alcohol precipitation steps. If you’ve ever carefully pipetted layers after phase separation, trying not to disturb the interface, you’ll know how easy it is to introduce inconsistency at this stage.
Zymo Research developed an innovative approach with the Direct-zol™ RNA Kits: you can now recover ultra-pure total RNA, including miRNA, directly from samples prepared with TRI Reagent® or similar in as little as 7 minutes using the kit.
This direct binding of RNA to the spin-column eliminates the need for chloroform extraction, phase separation, and labor-intensive alcohol precipitation steps.
Try it in your own workflow and experience:
Improve Consistency by Streamlining the Workflow
Phase separation introduces variability because it depends on precise handling at every step.
Chloroform is a hazardous reagent, and using it means that you have to move your samples over to the fume hood to work. As Direct-zol™ eliminates the need for chloroform, you can perform the entire workflow at your bench in a cleaner, safer way. By eliminating complex steps, Direct-zol™ helps standardize RNA extraction across samples and users.
This makes it easier to:
Generate High-quality RNA Ready for Downstream Applications
Direct-zol™ is designed to recover ultra-pure RNA suitable for demanding downstream applications—even from low-input samples. Because the workflow is shorter, there is less opportunity for degradation or variability.
With this kit, you can:
Because the workflow is shorter and avoids harsh steps like chloroform extraction and precipitation, there is less opportunity for degradation, loss, or variability.
Recover RNA Reliably from Low-input Samples Without Bias
Working with limited or precious samples makes RNA extraction even more challenging.
With traditional phase separation methods, low-input workflows often suffer from:
In fact, conventional extraction methods can introduce bias by selectively enriching certain RNA species, which can distort downstream analysis.
Direct-zol™ avoids these issues because RNA binds directly from TRI Reagent®-preserved samples, so you get:
This gives you greater confidence when working with precious material, where every preparation counts and accuracy matters.
Used by Researchers Working with Sensitive Samples and Developed by a Company Known for Practical Lab Solutions
Direct-zol™ is used in workflows where RNA quality and consistency are critical, including transcriptomics and other sensitive applications.
“Excellent for most RNA, including microRNA. Efficient, easy, time saver. In my opinion, the only column-based RNA extraction system that efficiently recovers small RNA (including miRNA).”
T.S., IGF
High-quality, intact, small and large RNA are efficiently recovered using a Direct-zol™ RNA kit compared to using a Supplier Q kit. RNA is DNA-free and ready for all downstream applications, including NGS.
Zymo Research is well known for developing tools that address real challenges faced at the bench. It also offers additional RNA purification tools for rapid isolation and downstream cleanup, allowing you to adapt your workflow as needed while maintaining sample quality.
Direct-zol™ is another breakthrough product that reflects this approach, simplifying a workflow step that is often unnecessarily complex.
Proven Spin-column Technology in Large-Scale RNA Testing
The technology behind Direct-zol™ has been shown to work reliably at scale.
In a global evaluation of an RT-PCR-based rabies test (LN34 assay), 2,978 samples from laboratories across Africa, Asia, the Americas, Europe, and the Middle East were processed using Direct-zol™ RNA Purification Kits.
By eliminating chloroform and phase separation, this approach enabled a standardized, easy-to-use RNA extraction workflow (bind–wash–elute) that could be performed consistently across labs, without requiring specialist expertise.
This allowed researchers to:
The assay demonstrated strong robustness, low variability, and high diagnostic sensitivity.
Test It in Your Own Workflow
The most reliable way to evaluate an RNA extraction method is to use it on your own samples.
With your free sample, you can:
Try a simpler RNA extraction workflow and see how removing phase separation affects your results.