Optimize PCR Amplification in Single-cell RNA-seq

Traditional thermocyclers require you to choose a cycle number before you know how your samples will behave. But different inputs require different cycling conditions. So you split samples across multiple runs, quantify libraries between PCR steps, and hope you haven’t over- or under-amplified.

Real-time PCR control technology improves library prep by automatically stopping amplification at the optimal point. 

In this app note, see how icon96™ with AutoNorm™ technology simplifies your workflow by:

• Reducing the total number of PCR cycles per sample, minimizing overamplification
• Eliminating pre-index PCR quantification for faster turnaround times
• Reducing hands-on time by 40 to 60%