Precision Ultramicrotomy: Application-driven Innovations for Sectioning Resin-embedded Samples

Precision ultramicrotomy plays a central role in many electron microscopy (EM) workflows, particularly those requiring ultrathin serial sections for transmission electron microscopy (TEM), array tomography, and correlative light and electron microscopy (CLEM).

Yet for many laboratories, sectioning remains a highly manual, skill-dependent bottleneck that is vulnerable to variability, compromising downstream imaging and analysis.

This free ebook brings together expert application notes, real-world workflows, and hard-won practical insight to help you achieve reproducible, high-quality ultrathin sectioning of resin-embedded biological samples and polymers, supporting scalable, serial-section-based 3D datasets.

Rather than a textbook, this resource is designed to give you clear, actionable guidance you can apply directly at the ultramicrotome, supporting better experimental planning, more consistent training outcomes, and faster troubleshooting when sectioning quality limits your results.

Download now to:
• Understand how section consistency is critical for EM data quality, and why small variations in thickness can distort measurements and 3D reconstruction in section-based workflows
• Apply practical trimming, alignment, and cutting strategies to produce clean, reproducible ultrathin sections, reducing artifacts, stabilizing ribbons, and minimizing user-to-user variability when working with resin-embedded samples
• Accurately target regions of interest using in-resin fluorescence and 3D micro-computed tomography workflows, allowing you to trim directly to defined target planes while reducing sample loss and preparation time
• Integrate ultramicrotomy into high-throughput EM workflows, supporting volume imaging, polymer analysis, and AI-assisted downstream image analysis through consistent, high-quality sectioning

Get your copy of Precision Ultramicrotomy today and apply proven ultramicrotomy strategies that support reproducible, high-quality EM data, developed in collaboration with Leica Microsystems.