qPCR Efficiency & Ct Reference Card

Every time you run a standard curve, you need to answer one question before proceeding: is this assay efficient enough to use? This card helps you answer that question in seconds.

What’s on the card:

• A slope-to-efficiency lookup table covering the full practical range, color-coded so you can see at a glance whether your result is ideal, acceptable, borderline, or a hard stop.
• A three-zone efficiency strip — below 90%, 90–110%, above 110% — with the most likely cause and the specific action for each zone.
• A Ct / Cq range guide covering all four scenarios from baseline-risk territory below cycle 15 to the stochastic limit above 35, with what each means and what to do.
• And much, much more!