Bitesize Bio’s qPCR Helper Pack

Setting up qPCR is full of small calculations, judgment calls, and protocol details that are easy to get wrong.

Our qPCR Helper Pack gives you four practical tools built by Bitesize Bio: spreadsheet calculators to take the maths out of oligo prep and fold change analysis, a bench-ready troubleshooting card for when results look wrong, and a curated guide to the 11 papers that underpin rigorous qPCR practice.

Download the pack today and stop basic qPCR errors in their tracks.

• Tool 1 — qPCR Oligo Prep Helper (Excel) Four linked sheets covering resuspension & dilution calculations (up to 20 primers), freeze-thaw aliquot planning, a pre-session prep checklist, and a reaction stock tracker that flags low-stock primers automatically.
• Tool 2 — ΔΔCt Calculator (Excel) Enter your four average Ct values and get ΔCt, ΔΔCt, and fold change instantly. Includes primer efficiency checks, automatic warnings for Ct > 35 or efficiency outside 90–110%, and a batch analysis sheet for up to 20 samples.
• Tool 3 — qPCR Troubleshooting Reference Card (PDF) A bench-ready single-page reference covering the ΔΔCt formula, five pre-calculation checks, symptom/cause/fix for the most common result problems, and the mistakes that protocols don’t warn you about.
• Tool 4 — 11 qPCR Papers Every Researcher Should Know (PDF) Plain-English summaries of the essential literature on qPCR best practices, housekeeping gene validation, and quantification methods — from MIQE and geNorm to the Livak and Pfaffl methods.