RT-qPCR Method Selection & Troubleshooting Card

Most RT-qPCR problems are decided before amplification starts. This card helps you make those decisions before you commit your RNA and gives you a structured path out when a run still goes wrong.

Download now to:

• • Choose one-step or two-step confidently using a six-factor decision table before you commit your RNA
  • Catch setup risks before you run — priming strategy, enzyme choice for GC-rich targets, and reference gene validation, all flagged on Side 1
  • Work through a failing run systematically — follow the symptom flowchart to a clear next action, including how to isolate whether RT or qPCR is the failing step
  • Keep a laminate-ready reference at the bench for no amplification, inconsistent Ct, unexpected melt curve behavior, and low yield