Western Blot Troubleshooting Card

Did you know that your Western blot may be failing due to an interpretation mistake, rather than a dodgy protocol?

For example, the absence of bands might look like an antibody problem, but did you definitely confirm that the transfer step worked?

Download this troubleshooting card FREE and access:

• • 12 troubleshooting scenarios, each with a concrete fix, e.g., no band, faint band, multiple bands, high background, uneven transfer, and *shudders* smearing
  • Band interpretation table
  • What the protocol doesn’t tell you (including why your loading control might be lying to you!)