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The Fundamentals of qPCR and RT-qPCR
Your handbook to becoming an expert at indispensable molecular biology techniques
qPCR and RT-qPCR are everywhere in modern molecular biology. But getting reliable, reproducible results means understanding what’s really going on, not just following a protocol.
Whether you’re setting up your first run or want to sharpen your fundamentals, this free handbook walks you through qPCR and RT-qPCR from the ground up.
What’s inside
- How to nail the basics of qPCR set-up
- The advantages of qPCR and RT-qPCR, and when each earns its place
- The difference between One-Step and Two-Step qRT-PCR — clearly explained
- How to choose the right PCR application for your experiment