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Get High Transient Expression Yields, From a Plate to a 5 L Flask
Every culture vessel mixes, breathes, and grows differently, so the shake speed and working volume that work in one format can cost you growth, viability, and titer in the next.
The Gibco™ Expi293™ PRO Expression System was developed to deliver high protein yields, even for challenging and traditionally low-yield targets, with the speed and reliability to run across many vessel formats.
This application note provides tested settings for running it across deep-well plates, 50 mL and 600 mL bioreactor tubes, and 7 L shake flasks, at 5 mL up to 5 L, with titers benchmarked against a standard shake flask.
Download now to:
- Get as much protein as a shake flask, or up to 9 percent more
- Keep your titer steady when you switch plates, tubes, and flasks
- Move from screening to 5 L without re-optimizing your transfection
- Save medium and reagent at every scale
- Avoid failed runs from foaming, wet filters, or detached clamps
Performance with extra-high 24 deep-well plates (left) and with 6 deep-well plates (right).